3D parallel pulsed chaos LiDAR system.

We propose and experimentally demonstrate a parallel pulsed chaos light detection and ranging (LiDAR) system with a high peak power, parallelism, and anti-interference. The system generates chaotic microcombs based on a chip-scale Si3N4 microresonator. After passing through an acousto-optic modulator, the continuous-wave chaotic microcomb can be transformed into a pulsed chaotic microcomb, in which each comb line provides pulsed chaos. Thus, a parallel pulsed chaos signal is generated. Using the parallel pulsed chaos as the transmission signal of LiDAR, we successfully realize a 4-m three-dimensional imaging experiment using a microelectromechanical mirror for laser scanning. The experimental results indicate that the parallel pulsed chaos LiDAR can detect twice as many pixels as direct detection continuous wave parallel chaos LiDAR under a transmission power of -6 dBm, a duty cycle of 25%, and a pulse repetition frequency of 100 kHz. By further increasing the transmission power to 10 dBm, we acquire an 11 cm × 10 cm image of a target scene with a resolution of 30 × 50 pixels. Finally, the anti-jamming ability of the system is evaluated, and the results show that the system can withstand interferences of at least 15 dB.

Optimizing auxiliary laser heating for Kerr soliton microcomb generation.

Auxiliary laser heating has become a widely adopted method for Kerr soliton frequency comb generation in optical microcavities, thanks to its reliable and easy-to-achieve merits for solving the thermal instability during the formation of dissipative Kerr solitons. Here, we conduct optimization of auxiliary laser heating by leveraging the distinct loss and absorption characteristics of different longitudinal and polarization cavity modes. We show that even if the auxiliary and pump lasers enter orthogonal polarization modes, their mutual photothermal balance can be efficient enough to maintain a cavity thermal equilibrium as the pump laser enters the red-detuning soliton regime, and by choosing the most suitable resonance for the auxiliary and pump lasers, the auxiliary laser power can be reduced to 20% of the pump laser and still be capable of warranting soliton generation. Moreover, we demonstrate soliton comb generation using integrated laser modules with a few milliwatt on-chip pump and auxiliary powers, showcasing the potential for further chip integration of the auxiliary laser heating method.

The Construction of lncRNA/circRNA-miRNA-mRNA Networks Reveals Functional Genes Related to Growth Traits in Schima superba.

Schima superba is a precious timber and fire-resistant tree species widely distributed in southern China. Currently, there is little knowledge related to its growth traits, especially with respect to molecular breeding. The lack of relevant information has delayed the development of modern breeding. The purpose is to identify probable functional genes involved in S. superba growth through whole transcriptome sequencing. In this study, a total of 32,711 mRNAs, 525 miRNAs, 54,312 lncRNAs, and 1522 circRNAs were identified from 10 S. superba individuals containing different volumes of wood. Four possible regulators, comprising three lncRNAs, one circRNA, and eleven key miRNAs, were identified from the regulatory networks of lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA to supply information on ncRNAs. Several candidate genes involved in phenylpropane and cellulose biosynthesis pathways, including Ss4CL2, SsCSL1, and SsCSL2, and transcription factors, including SsDELLA2 (SsSLR), SsDELLA3 (SsSLN), SsDELLA5 (SsGAI-like2), and SsNAM1, were identified to reveal the molecular regulatory mechanisms regulating the growth traits of S. superba. The results not merely provide candidate functional genes related to S. superba growth trait and will be useful to carry out molecular breeding, but the strategy and method also provide scientists with an effective approach to revealing mechanisms behind important economic traits in other species.

Massive and parallel 10 Tbit/s physical random bit generation with chaotic microcomb.

Ultrafast physical random bit (PRB) generators and integrated schemes have proven to be valuable in a broad range of scientific and technological applications. In this study, we experimentally demonstrated a PRB scheme with a chaotic microcomb using a chip-scale integrated resonator. A microcomb contained hundreds of chaotic channels, and each comb tooth functioned as an entropy source for the PRB. First, a 12 Gbits/s PRB signal was obtained for each tooth channel with proper post-processing and passed the NIST Special Publication 800-22 statistical tests. The chaotic microcomb covered a wavelength range from 1430 to 1675 nm with a free spectral range (FSR) of 100 GHz. Consequently, the combined random bit sequence could achieve an ultra-high rate of about 4 Tbits/s (12 Gbits/s × 294 = 3.528 Tbits/s), with 294 teeth in the experimental microcomb. Additionally, denser microcombs were experimentally realized using an integrated resonator with 33.6 GHz FSR. A total of 805 chaotic comb teeth were observed and covered the wavelength range from 1430 to 1670 nm. In each tooth channel, 12 Gbits/s random sequences was generated, which passed the NIST test. Consequently, the total rate of the PRB was approximately 10 Tbits/s (12 Gbits/s × 805 = 9.66 Tbits/s). These results could offer potential chip solutions of Pbits/s PRB with the features of low cost and a high degree of parallelism.

Comparative transcriptomics of Pinus massoniana organs provides insights on terpene biosynthesis regulation.

Terpenoids are the most important natural products collected from conifer species. However, the molecular mechanisms and core factors underlying terpenoid biosynthesis in Pinus massoniana remain unclear. To clarify these mechanisms, this study aimed to identify potential genes that might participate in the terpenoid biosynthesis of P. massoniana. In this study, single molecule real-time (SMRT) sequencing and expression analysis were used to confirm the expression patterns of genes involved in the cones, immature needles, mature needles, immature branches, and mature branches of P. massoniana. A total of 31,331 lncRNAs and 71,240 mRNAs were identified from these organs, and the greatest number of differentially expressed genes (DEGs) was discovered between needles and branches. Weighted gene coexpression network analysis (WGCNA) classified all expressed genes into nine typical modules with 11 kinds of transcription factors (TFs), namely, AP2-ERF, ARF, AUX-IAA, C2H2, Dof, F-box, SBP, WRKY, bHLH, bZIP, and GRAS, and seven kinds of functional genes, namely, ABC transporter, cellulose synthase (CesA), leucine-rich repeats (LRR), cytochrome P450 (CYT P450), pathogenesis-related protein (PR), terpene synthase (TPS), and chlorophyllase enzyme. A molecular network was constructed for hub genes, TFs, and functional genes in three modules. The potential function of eight candidate genes, including PmbHLH2, PmERF1, PmRGA, PmGAI, PmbZIP1, PmLOB1, PmMADS1, and PmMYB1, was validated through correlation analysis between terpenoid contents and expression levels, subcellular localization, and transcriptional activation activity, which provides us with probable regulators of terpenoid biosynthesis in conifers.

Phase noise of Kerr soliton dual microcombs.

Dissipative Kerr soliton microcombs are believed to be a promising technique to build a dual-comb source for applications including precision laser metrology, fast laser spectroscopy, and high-speed optical signal processing. In this Letter, we conduct a detailed experimental investigation on the phase coherence between two on-chip Kerr soliton microcombs, where the underlying physical and technical origins that lead to the mutual phase noise between microcombs are analyzed. Moreover, the techniques of 2-point locking and optical frequency division are explored to enhance the dual-microcomb phase coherence, and we demonstrate the best phase noise down to -50 dBc/Hz at 1-Hz offset, -90 dBc/Hz at 1-kHz offset, and -120 dBc/Hz at 1-MHz offset. Our study provides a basic reference for both fundamental studies and practical applications of Kerr soliton dual microcombs that entail high mutual phase coherence.

TCM Treatment and Drug Co-Occurrence Analysis of Psoriasis.

Psoriasis is a long-term immune-mediated disease. Patients with a long history and slow progress are more common, and its treatment is difficult. This study proposes to use traditional Chinese medicine to treat psoriasis. Through the follow-up of all participants for 12 and 24 weeks, a large number of comparative experiments effectively verify the effectiveness of the method proposed in this study. The research results of this study can provide some reference ideas for follow-up research.

Wavelength-division multiplexing communications using integrated soliton microcomb laser source.

In this Letter, we report an investigation of the feasibility and performance of wavelength-division multiplexed (WDM) optical communications using an integrated perfect soliton crystal as the multi-channel laser source. First, we confirm that perfect soliton crystals pumped directly by a distributed-feedback (DFB) laser self-injection locked to the host microcavity has sufficiently low frequency and amplitude noise to encode advanced data formats. Second, perfect soliton crystals are exploited to boost the power level of each microcomb line, so that it can be directly used for data modulation, excluding preamplification. Third, in a proof-of-concept experiment, we demonstrate seven-channel 16-quadrature amplitude modulation (16-QAM) and 4-level pulse amplitude modulation (PAM4) data transmissions using an integrated perfect soliton crystal as the laser carrier; excellent data receiving performance is obtained for various fiber link distances and amplifier configurations. Our study reveals that fully integrated Kerr soliton microcombs are viable and advantageous for optical data communications.

Transcriptomic and metabolomic analyses reveal several critical metabolic pathways and candidate genes involved in resin biosynthesis in Pinus massoniana.

Pine resin, which typically consists of terpenoids, is a natural product used in various industrial applications. Oleoresin can be obtained from the xylem tissue by wounding the stem bark. Pinus massoniana (masson pine) is an important resin-tapping tree species that originated in southern China. Masson pines with different genetic backgrounds typically have different resin-yielding capacities (RYCs). However, the mechanisms underlying high resin yield in masson pines are unclear. The aim of this study was to identify the possible genetic regulation pathways and functional genes that influence the resin yield. In this study, we conducted transcriptomic and metabolomic studies of masson pine secondary xylem with high, medium, and low RYCs. A total of 230,068 unigenes and 3894 metabolites were identified from the tissue of the secondary xylem. Several differentially expressed regulation factors, including WRKY, bHLH, and ERF, and functional genes such as PKc and LRR-RLKs, were identified among these masson pines. The Kyoto Encyclopedia of Genes and Genomes pathways were mainly focused on diterpenoid biosynthesis, plant hormone signal transduction, and ABC transporters. Furthermore, integration of the transcriptomic and metabolomic data indicated that the PKc- and LRR-RLK-related regulatory and metabolic pathways may play critical roles in the biosynthesis of terpenoids. These above results improve our understanding of the biosynthesis mechanism of oleoresin in P. massoniana and facilitate further research work into the functional analysis of these candidate genes.

Core set construction and association analysis of Pinus massoniana from Guangdong province in southern China using SLAF-seq.

Germplasm resource collection and utilization are important in forestry species breeding. High-through sequencing technologies have been playing increasing roles in forestry breeding. In this study, specific-locus amplified fragment sequencing (SLAF-seq) was employed to analyze 149 masson pine (Pinus massoniana) accessions collected from Guangdong in China. A large number of 471,660 SNPs in the total collection were identified from 599,164 polymorphic SLAF tags. Population structure analysis showed that 149 masson pines could not be obviously divided into subpopulations. Two core sets, containing 29 masson pine accessions for increasing resin and wood yield respectively, were obtained from the total collection. Phenotypic analyses of five traits showed abundant variations, 25 suggestive and 9 significant SNPs were associated with the resin-yielding capacity (RYC') and volume of wood (VW) using EMMAX and FaST-LMM; 22 suggestive and 11 significant SNPs were associated with RYC' and VW using mrMLM and FASTmrMLM. Moreover, a large number of associated SNPs were detected in trait HT, DBH, RW and RYC using mrMLM, FASTmrMLM, FASTmrEMMA and ISIS EM-BLASSO. The core germplasm sets would be a valuable resource for masson pine improvement and breeding. In addition, the associated SNP markers would be meaningful for masson pine resource selection.

The Exploration and Confirmation of the Maximum Mass Sensitivity of Quartz Crystal Microbalance.

After the advent of the quartz crystal microbalance (QCM) technology, various QCM-based sensing systems have got certain applications in many science and technology fields and resulted in dramatic progress in these fields. The core advantage of QCM is its high mass sensitivity which leads to high accuracy and low detection limit. For a QCM, the mass sensitivity is determined by the diameter and thickness of the electrode to a certain extent when the frequency of the quartz wafer is already determined. Theoretical approximate calculation reveals that there is an optimum electrode diameter corresponding to the maximum sensitivity. This is different from the traditional opinion that the smaller the electrode, the higher the mass sensitivity. A plating experiment was carried out using 28 QCMs with different electrode diameters, and the experimental results verified the existence of the optimum diameter. This study is helpful to obtain higher mass sensitivity by optimizing electrode parameters.

Quartz Crystal Microbalance with Approximately Uniform Sensitivity Distribution.

The nonuniformity of QCMs' mass sensitivity distribution is a disadvantage to practical applications. Through theoretical calculations, we found that common ring electrode QCMs could obtain approximately uniform sensitivity distribution by carefully selecting the inner and outer diameters and mass loading factor of the electrode. A series of experiments were carried out using 10 MHz ring electrode QCMs with an inner diameter of 2 mm, an outer diameter of 5 mm, and a loading factor R of 0.0044. The experimental results proved that its mass sensitivity distribution is approximately uniform. This special designed ring electrode QCMs is suitable and convenient for highly accurate measurements.

The association of hormone signalling genes, transcription and changes in shoot anatomy during moso bamboo growth.

Moso bamboo is a large, woody bamboo with the highest ecological, economic and cultural value of all the bamboo types and accounts for up to 70% of the total area of bamboo grown. However, the spatiotemporal variation role of moso bamboo shoot during growth period is still unclear. We found that the bamboo shoot growth can be divided into three distinct periods, including winter growth, early growth and late growth based on gene expression and anatomy. In the early growth period, lateral buds germinated from the top of the bamboo joint in the shoot tip. Intercalary meristems grew vigorously during the winter growth period and early growth period, but in the late growth period, mitosis in the intercalary meristems decreased. The expression of cell cycle-associated genes and the quantity of differentially expressed genes were higher in early growth than those in late growth, appearing to be influenced by hormonal concentrations. Gene expression analysis indicates that hormone signalling genes play key roles in shoot growth, while auxin signalling genes play a central role. In situ hybridization analyses illustrate how auxin signalling genes regulate apical dominance, meristem maintenance and lateral bud development. Our study provides a vivid picture of the dynamic changes in anatomy and gene expression during shoot growth in moso bamboo, and how hormone signalling-associated genes participate in moso bamboo shoot growth.

A Practical Model of Quartz Crystal Microbalance in Actual Applications.

A practical model of quartz crystal microbalance (QCM) is presented, which considers both the Gaussian distribution characteristic of mass sensitivity and the influence of electrodes on the mass sensitivity. The equivalent mass sensitivity of 5 MHz and 10 MHz AT-cut QCMs with different sized electrodes were calculated according to this practical model. The equivalent mass sensitivity of this practical model is different from the Sauerbrey's mass sensitivity, and the error between them increases sharply as the electrode radius decreases. A series of experiments which plate rigid gold film onto QCMs were carried out and the experimental results proved this practical model is more valid and correct rather than the classical Sauerbrey equation. The practical model based on the equivalent mass sensitivity is convenient and accurate in actual measurements.

The Resistance-Amplitude-Frequency Effect of In-Liquid Quartz Crystal Microbalance.

Due to the influence of liquid load, the equivalent resistance of in-liquid quartz crystal microbalance (QCM) increases sharply, and the quality factor and resonant frequency decreases. We found that the change in the resonant frequency of in-liquid QCM consisted of two parts: besides the frequency changes due to the mass and viscous load (which could be equivalent to motional inductance), the second part of frequency change was caused by the increase of motional resistance. The theoretical calculation and simulation proved that the increases of QCM motional resistance may indeed cause the decreases of resonant frequency, and revealed that the existence of static capacitance was the root cause of this frequency change. The second part of frequency change (due to the increases of motional resistance) was difficult to measure accurately, and may cause great error for in-liquid QCM applications. A technical method to reduce the interference caused by this effect is presented. The study contributes to the accurate determination of the frequency and amplitude change of in-liquid QCM caused by liquid load, which is significant for the QCM applications in the liquid phase.

Analysis of MADS-Box Gene Family Reveals Conservation in Floral Organ ABCDE Model of Moso Bamboo (Phyllostachys edulis).

Mini chromosome maintenance 1, agamous, deficiens, and serum response factor (MADS)-box genes are transcription factors which play fundamental roles in flower development and regulation of floral organ identity. However, till date, identification and functions of MADS-box genes remain largely unclear in Phyllostachys edulis. In view of this, we performed a whole-genome survey and identified 34 MADS-box genes in P. edulis, and based on phylogeny, they were classified as MIKCC, MIKC∗, Mα, and Mß. The detailed analysis about gene structure and motifs, phylogenetic classification, comparison of gene divergence and duplication are provided. Interestingly, expression patterns for most genes were found similar to those of Arabidopsis and rice, indicating that the well-established ABCDE model can be applied to P. edulis. Moreover, we overexpressed PheMADS15, an AP1-like gene, in Arabidopsis, and found that the transgenic plants have early flowering phenotype, suggesting that PheMADS15 might be a regulator of flowering transition in P. edulis. Taken together, this study provides not only insightful comprehension but also useful information for understanding the functions of MADS-box genes in P. edulis.

Genome-wide analysis and expression characteristics of small auxin-up RNA (SAUR) genes in moso bamboo (Phyllostachys edulis).

Moso bamboo (Phyllostachys edulis) is well known for its rapid shoot growth. Auxin exerts pleiotropic effects on plant growth. The small auxin-up RNA (SAUR) genes are early auxin-responsive genes involved in plant growth. In total, 38 SAUR genes were identified in P. edulis (PheSAUR). A comprehensive overview of the PheSAUR gene family is presented, including the gene structures, phylogeny, and subcellular location predictions. A transcriptome analysis indicated that 37 (except PheSAUR18) of the PheSAUR genes were expressed during shoot growth process and that the PheSAUR genes were differentially expressed. Furthermore, quantitative real-time PCR analysis indicated that all of the PheSAUR genes could be induced in different tissues of seedlings and that 37 (except PheSAUR41) of the PheSAUR genes were up-regulated after indole-3-acetic acid (IAA) treatment. These results reveal a comprehensive overview of the PheSAUR gene family and may pave the way for deciphering their functions during bamboo development.